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A schematic representation of the design of an aptamer-DNAzyme conjugated biosensor for <t>microarray</t> screening. The designed biosensor comprises an aptamer region (red), a DNAzyme region (blue), an internal loop region (cyan), two block sequences (gray) for the aptamer and the DNAzyme, and a linker region, immobilized on the microarray electrode.
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A schematic representation of the design of an aptamer-DNAzyme conjugated biosensor for microarray screening. The designed biosensor comprises an aptamer region (red), a DNAzyme region (blue), an internal loop region (cyan), two block sequences (gray) for the aptamer and the DNAzyme, and a linker region, immobilized on the microarray electrode.

Journal: Sensors (Basel, Switzerland)

Article Title: An Aptamer-Based Biosensor for Direct, Label-Free Detection of Melamine in Raw Milk

doi: 10.3390/s18103227

Figure Lengend Snippet: A schematic representation of the design of an aptamer-DNAzyme conjugated biosensor for microarray screening. The designed biosensor comprises an aptamer region (red), a DNAzyme region (blue), an internal loop region (cyan), two block sequences (gray) for the aptamer and the DNAzyme, and a linker region, immobilized on the microarray electrode.

Article Snippet: The 1200 unique sequences, 1180 for sensor screening, and 20 as control sequences, were randomly located on the 12K ElectraSense microarray chip purchased from Recenttec K.K. (Tokyo, Japan), with ten replicates.

Techniques: Microarray, Blocking Assay